qPCR Scientist III, Molecular Biology

Key Responsibilities 

• Lead the design, optimization, and validation of qPCR assays for gene expression, genotyping, and molecular diagnostics applications. 

• Perform RNA/DNA extraction, cDNA synthesis, and nucleic acid quantification using spectrophotometric and fluorometric methods. 

• Validate assay performance, including sensitivity, specificity, efficiency, and reproducibility. 

• Analyze and interpret qPCR data using software such as Applied Biosystems QuantStudio, Bio-Rad CFX Manager, or equivalent platforms. 

• Troubleshoot technical issues and propose innovative solutions to improve assay performance. 

• Maintain accurate and detailed experimental records, laboratory notebooks, and data reports. 

• Collaborate cross-functionally with R&D, QC, and bioinformatics teams to support product development or experimental studies. 

• Ensure compliance with laboratory safety protocols, quality management systems, and regulatory standards (e.g., ISO, GLP, or CLIA, as applicable). 

• Present experimental findings in technical meetings and contribute to scientific documentation and publications. 

• Mentor junior scientists and research associates, providing technical guidance and fostering a culture of collaboration and continuous improvement. 

Qualifications 

Education: Master’s or Ph.D. in Molecular Biology, Biochemistry, Genetics, or a related life sciences discipline. Experience requirements will vary based on degree. 

Experience: 

• Minimum of 2–5 years of hands-on experience performing qPCR (probe based chemistry preferred), including multiplexed qPCR reactions. 

• Prior experience with assay development, validation, and data analysis. 

• Familiarity with nucleic acid extraction instruments or workflows is expected. 

• Additional experience in NGS is a plus. 

Technical Skills: 

• In-depth understanding of molecular biology principles, qPCR kinetics, and assay troubleshooting. 

• Strong analytical and problem-solving skills with a data-driven mindset. 

• Strong understanding of qPCR principles, primer/probe design, and amplification kinetics. 

• Proficiency in data analysis, statistical interpretation, and report generation. 

• Experience working under quality and regulatory standards preferred. 

Soft Skills: 

• Excellent organizational, communication, and problem-solving skills. 

• Ability to work independently as well as collaboratively in a multidisciplinary environment. 

• Strong attention to detail and scientific curiosity. 

Preferred Qualifications 

• Familiarity with bioinformatics tools for assay design 

• Experience with digital PCR (dPCR) 

• Knowledge of automation and data management systems.